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Chemical collection and stock control for UK schools, universities and hospitals

Bromothymol blue (CAS 76-59-5): storage and disposal

Bromothymol blue is the indicator that is yellow in acid, green near neutral and blue in alkali, and it is a fixture of school and college prep rooms for carbon dioxide and respiration practicals. Stock is held as powder and as a 0.04% solution. It is not normally classified as hazardous, but it remains a chemical waste that needs accurate description.

CAS number
76-59-5
Form
Solid
Storage class
General
Usual EWC code, laboratory stock
16 05 09

What to do this week

  1. Without moving anything that looks damaged, check the label is readable and write down the opened date if it is not already there.
  2. Check it is stored as general stock, and apart from anything listed under "Do not store with" on this page. If nothing is listed, that does not prove it is safe with everything.
  3. If you no longer need it, add it to your disposal list so it can be included in a collection.

Safe storage in a school or lab

In a school prep room the indicator bottles tend to live together on one shelf near the burettes, which is a sensible place for the dropper bottles but not for the powder, since a splash or a leak from an acid or alkali bottle nearby would change colour in the stock. Keep powder and solution in closed bottles on a general shelf, separate from food supplies, and keep dropper bottles upright in a tray. Put the opening date on the 0.04% solution, as the colour may fade and the solvent can escape through a loose cap, so stock that is years old has changed without anyone looking. A university store follows the same pattern.

How to tell it has deteriorated

A faded or smudged label is often the first thing seen, because indicator drips run down the side of the bottle and bleach the print. Inside, the colour of the solution may fade, and the level may drop where solvent has escaped through the cap, leaving a dried blue or yellow ring around the neck. A bottle that is meant to be blue in alkaline stock but has gone green or yellow has probably taken up acid, possibly carbon dioxide from the air through a poor seal. Powder should be loose, so damp or caked powder suggests moisture has entered. Stock with an unknown date of receipt is awkward to describe.

How it is disposed of

We arrange this by specialist collection. We do not publish a drain or general waste route for any chemical. It is normally packed in a non-regulated or low hazard laboratory pack with compatible chemicals.

A weak solution of a dye still needs to be described properly. Bromothymol blue is not normally classified as hazardous, so the ordinary waste transfer note usually covers it, but it remains a chemical waste, usually under code 16 05 09, and must be recorded accurately by form, strength and volume. Powder and solution are listed separately, and the dropper bottles are packed upright in a lab pack with other compatible items. Colour fading or solvent loss does not change that. Stock should not be rinsed into a sink or drain, however pale the solution, and nobody should try to clear it by diluting it.

Waste codes for Bromothymol blue
Usual EWC code, laboratory stock16 05 09
Usually classed as hazardous wasteNo

The code depends on where the waste came from and what it contains, and classifying it is the producer's duty. Use the waste code finder to see why.

Common quantities

Usually held as: solid, 0.04% solution.

Tell us the container size and how many you hold. An estimate is enough to start with, and some items cannot be priced until someone has looked at them.

Where it is commonly held

Questions

Our bromothymol blue has changed from blue to green in the bottle. Why?

The dye is yellow in acid and blue in alkali, so a drift towards green or yellow suggests the stock has taken up acid, possibly carbon dioxide through a poor cap. It is a sign of age, not a hazard signal.

Is bromothymol blue classed as hazardous waste?

It is not normally classified as hazardous, so a waste transfer note usually applies. It is still a chemical waste, usually under 16 05 09, that needs accurate description, and it is not for the sink or the bin.

How should the 0.04% solution be recorded?

Give the product name, the stated strength, the approximate volume, the bottle type and the room. Mention if the level has dropped or the colour has faded, since the collector groups liquids separately from powder.

Guidance only. Check your own risk assessment and your supplier's safety data sheet.

Hazard data source: PubChem GHS Classification, retrieved 31 Oct 2026. Formula C27H28Br2O5S. EC number 200-971-2.

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