Coomassie brilliant blue R-250 (CAS 6104-59-2): storage and disposal
Protein bands on a gel turn blue with Coomassie brilliant blue R-250, so university and pathology research labs keep it as a powder in the stock cupboard. It is not classified as hazardous on the sources we checked, but it is still a chemical waste that has to be described accurately. The powder is only half the picture, because gel staining solutions made from it are usually a different waste with a different description.
- CAS number
- 6104-59-2
- Form
- Solid
- Storage class
- General
- Usual EWC code
- 16 05 09
What to do this week
- Check the label is readable and write down the opened date if it is not already there.
- Check it is stored as general stock and apart from the items listed under "Do not store with".
- If you no longer need it, add it to your disposal list so it goes on the next collection.
Safe storage in a school or lab
Powder stays in its original jar in a dry, labelled, well organised store, because it is general stock and needs no cabinet. Date it on arrival, and keep the jar apart from the used staining solutions, which tend to collect in bottles beside the gel tank. Those bottles are a separate stock with their own labels, and the powder jar's label does not describe them. A research lab with several users often ends up with two or three half-used jars, so keep one record and consolidate nothing, since combining jars hides their age. A tray under the jar shows blue dust at once.
How to tell it has deteriorated
Sound powder is dry and runs freely behind a clean thread. Blue dust on the shoulder of the jar, a blue-stained label or marks on the shelf below show the closure has failed, and a label stained until unreadable leaves an unknown jar. Lumps, a damp texture or a hard cake mean moisture has entered. This dye gives no specific sign of age, so beyond these checks it gives few visual clues. Rely on the paperwork: no date of receipt, duplicate jars with no record or a stock entry that no longer matches. An unlabelled blue jar is listed unknown and left sealed.
How it must be disposed of
By specialist collection only. It never goes down the drain or into general waste. It is normally packed in a non-regulated or low hazard laboratory pack with compatible chemicals.
Never put the powder into a sink or general waste, and do not try to treat a stained jar first. Although it is not classified as hazardous on the sources we checked, it stays a chemical waste needing an accurate description. List the powder jars separately from any used gel staining solutions, since the solutions are described by what they contain, usually with solvents, and not by the dye. A university or laboratory store uses 16 05 09 for the powder, and a human healthcare pathology service uses 18 01 07. Keep each jar sealed and upright.
| EWC code, laboratory chemicals | 16 05 09 |
|---|---|
| Human healthcare setting | 18 01 07 |
| Hazardous waste | No |
The code depends on where the waste came from and what it is mixed with. Use the waste code finder to see why.
Common quantities we collect
Usually held as: solid.
Part-used bottles and full containers are both fine. Tell us the size and how many, and an estimate is enough for a first price.
Where it is commonly held
Questions
Is used Coomassie gel stain the same waste as the powder?
No. Used staining solutions are made up with other ingredients and are described by what they contain, so list them as separate items from the powder jar and name the contents from the label.
Can we merge half-used jars of Coomassie to save space?
No. Merging hides the age of each jar and makes the contents harder to describe. Leave them as they are, labelled and dated, and list them individually.
Is Coomassie brilliant blue R-250 hazardous waste?
It is not classified as hazardous on the sources we checked, but it is still chemical waste that has to be described accurately and collected, not put into general waste.
Which code applies to a pathology research lab?
A human healthcare pathology service uses 18 01 07 for the powder, while a university or laboratory store uses 16 05 09. Give the setting when you request collection.
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