Eosin Y (CAS 17372-87-1): storage and disposal
Pink-red and fluorescent in the bottle, eosin Y is the counterstain on almost every haematoxylin and eosin staining line, so histology and pathology rooms hold it as powder and as 1% solution, with teaching labs holding smaller amounts. It is classified for serious eye irritation. The solution is often made up in alcohol, and that choice, together with how much sediment has formed, decides how a bottle is described when it leaves.
- CAS number
- 17372-87-1
- Form
- Solid
- Storage class
- General
- Signal word
- Warning
- Usual EWC code
- 16 05 06*
Harmful or irritant
What to do this week
- Check the label is readable and write down the opened date if it is not already there.
- Check it is stored as general stock and apart from the items listed under "Do not store with".
- If you no longer need it, add it to your disposal list so it goes on the next collection.
Safe storage in a school or lab
Powder sits happily on a dry general shelf, capped and dated. The bottles that need thought are the working solutions on a histology bench, because eosin is often held in alcohol, which adds flammability to the liquid. Keep those away from heat and ignition sources, and away from any oxidiser, even though the powder has no such restriction. Make sure the staining line reservoir is not topped up from a mixed bottle with another reagent, because the label then no longer describes the contents. A tray under bottles of 1% solution makes drips obvious, since the pink-red colour spreads quickly on white shelving and a paper label.
How to tell it has deteriorated
A clear, evenly coloured solution is what normal looks like. With age a precipitate forms, seen as a dark sediment on the bottom, flecks floating in the liquid or a crust round the neck and cap thread where the solution has dried. A bottle that has lost level, or whose lid has dried to a pink crust, has been losing solvent through the seal. The powder shows fewer signs, but caking or a damp, clumped look means moisture has entered, and pink dust on the cap points to a poor closure. Treat a bottle with a heavy sediment, a loose or glued lid or an unreadable label as stock to be listed rather than topped up and reused.
How it must be disposed of
By specialist collection only. It never goes down the drain or into general waste. It is normally packed in a non-regulated or low hazard laboratory pack with compatible chemicals.
Group eosin by form: powder in its original jar, and solutions by strength and by whether they are alcohol based, because that affects how they are packed with other items in a lab pack. The code that fits a school, college or university store is 16 05 06*, while human pathology and histology services clearing stock may use 18 01 06*. Note any heavy sediment or crusted lids on the request, since the carrier needs to know a closure may not hold. Pouring leftovers down a sink is not allowed, and a rinse of a pink bottle does not make it fit for general waste.
| EWC code, laboratory chemicals | 16 05 06* |
|---|---|
| Discarded organic chemical, not a laboratory pack | 16 05 08* |
| Human healthcare setting | 18 01 06* |
| Hazardous waste | Yes |
The code depends on where the waste came from and what it is mixed with. Use the waste code finder to see why.
Common quantities we collect
Usually held as: solid, 1% solution.
Part-used bottles and full containers are both fine. Tell us the size and how many, and an estimate is enough for a first price.
Where it is commonly held
Hazard statements
- H319 Causes serious eye irritation.
Precautionary statements
- P264+P265 Wash hands and exposed skin thoroughly after handling. Do not touch eyes.
- P280 Wear protective gloves, protective clothing, eye protection and face protection.
- P305+P351+P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P337+P317 If eye irritation persists: Get medical help.
Substance as classified, dilutions may differ.
Questions
Why has my eosin Y developed a sediment?
A precipitate forming with age is a recognised behaviour of eosin solutions. It is not a reason to keep topping the bottle up. Note the sediment and the date, and list a heavily affected bottle for collection rather than carrying on with it.
Does an alcoholic eosin solution count as flammable stock?
Eosin held in alcohol adds flammability to the solution, so keep those bottles away from ignition sources and oxidisers and tell the collector it is alcohol based. The powder alone does not carry that extra concern.
What do we write for the strength of staining solutions?
Use what is on the bottle, such as 1% eosin, plus the solvent if you know it and the container size. If a mixed bottle has lost its label, say so and describe it as unknown so it can be assessed before packing.
Should powder and solution be listed together?
List them as separate lines. The powder is dry stock in a jar, while the solution is a liquid that may contain alcohol and a precipitate, and the two are packed differently for collection.
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