Methyl green (CAS 7114-03-6): storage and disposal
Green nuclei in the methyl green and pyronin method make this dye a staple of histology and pathology, and university stores hold it as powder. It irritates the skin, the eyes and the airways. Its formula includes zinc, so it is a metal-containing dye and not an organic one alone, which is worth telling the collector. Most holders keep one old jar, and the way that jar is described matters more than anything else.
- CAS number
- 7114-03-6
- Form
- Solid
- Storage class
- General
- Signal word
- Warning
- Usual EWC code
- 16 05 06*
Harmful or irritant
What to do this week
- Check the label is readable and write down the opened date if it is not already there.
- Check it is stored as general stock and apart from the items listed under "Do not store with".
- If you no longer need it, add it to your disposal list so it goes on the next collection.
Safe storage in a school or lab
Because its formula includes zinc, keep it in a closed, labelled jar in a dry general store, away from acids and from the reducing and oxidising shelves, and record on the jar that it is a zinc-containing dye. The powder irritates the airways, so avoid raising dust. If the cap thread is contaminated, leave the deposit undisturbed and record its condition. Date it on arrival. Methyl green is usually used alongside pyronin, which is a different dye with a different classification, so keep their jars and records apart even though the two are mixed at the bench. A tray beneath the jar will show spilled green powder at once.
How to tell it has deteriorated
Powder that is dry and runs freely, with a clean thread, is the normal state. A green dusting on the shoulder of the jar, a stained label or powder on the shelf shows the cap has been closing on grit or has failed. Lumps, a damp look and a hard cake mean moisture has entered. This dye gives no specific sign of age, so beyond these checks it gives few visual clues, and the paperwork has to carry the evidence: unreadable label, missing date of receipt, record that no longer matches. An unlabelled green jar should be left sealed and listed as unknown.
How it must be disposed of
By specialist collection only. It never goes down the drain or into general waste. It is normally packed in a non-regulated or low hazard laboratory pack with compatible chemicals.
How it leaves the site is by collection, and the description should say that the dye contains zinc, as well as the form, jar size and any damage to the lid. A university or laboratory store uses 16 05 06*, and a human healthcare pathology service clearing it uses 18 01 06*. Because it irritates skin, eyes and airways, a jar with powder spilled on the outside should be bagged on its own. It is not emptied into a sink, a drain or general waste, and a green stain on a bench is not a reason to rinse the jar. Keep it sealed and upright.
| EWC code, laboratory chemicals | 16 05 06* |
|---|---|
| Discarded organic chemical, not a laboratory pack | 16 05 08* |
| Human healthcare setting | 18 01 06* |
| Hazardous waste | Yes |
The code depends on where the waste came from and what it is mixed with. Use the waste code finder to see why.
Common quantities we collect
Usually held as: solid.
Part-used bottles and full containers are both fine. Tell us the size and how many, and an estimate is enough for a first price.
Where it is commonly held
Hazard statements
- H315 Causes skin irritation.
- H319 Causes serious eye irritation.
- H335 May cause respiratory irritation.
Precautionary statements
- P260 Do not breathe dust, fume, gas, mist, vapours or spray.
- P261 Avoid breathing dust, fume, gas, mist, vapours or spray.
- P264 Wash hands and exposed skin thoroughly after handling.
- P264+P265 Wash hands and exposed skin thoroughly after handling. Do not touch eyes.
- P271 Use only outdoors or in a well-ventilated area.
- P280 Wear protective gloves, protective clothing, eye protection and face protection.
- P301+P330+P331 IF SWALLOWED: Rinse mouth. Do NOT induce vomiting.
- P302+P352 IF ON SKIN: Wash with plenty of water.
- P302+P361+P354 IF ON SKIN: Take off immediately all contaminated clothing. Immediately rinse with water for several minutes.
- P304+P340 IF INHALED: Remove person to fresh air and keep comfortable for breathing.
- P305+P351+P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P305+P354+P338 IF IN EYES: Immediately rinse with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P316 Get emergency medical help immediately.
- P319 Get medical help if you feel unwell.
- P321 Specific treatment (see supplemental first aid instruction on this label).
- P332+P317 If skin irritation occurs: Get medical help.
- P337+P317 If eye irritation persists: Get medical help.
- P362+P364 Take off contaminated clothing. And wash it before reuse.
- P363 Wash contaminated clothing before reuse.
- P403+P233 Store in a well-ventilated place. Keep container tightly closed.
- P405 Store locked up.
- P501 Dispose of contents and container in accordance with local, regional, national and international regulations.
Substance as classified, dilutions may differ.
Questions
Why is the zinc content mentioned for methyl green?
Its formula includes zinc, so it is a metal-containing dye. Tell the collector this when you list it, alongside the form and container size, so it is grouped correctly.
Is methyl green the same as methyl violet or crystal violet?
No. They are different dyes with different classifications, and methyl green also contains zinc. Describe each by the name on its own label and never merge jars or records.
Can we keep an old jar for the methyl green pyronin stain?
Only while it is dated, labelled and sealed with dry powder and a matching record. Damp, caked or undated jars are better listed for collection.
Which waste code applies to a university histology store?
University and other laboratory stores use 16 05 06*, whereas a human healthcare pathology service uses 18 01 06*. State the setting when requesting collection.
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