Oil red O (CAS 1320-06-5): storage and disposal
Frozen sections stained for fat are where oil red O is used, in pathology, histology and university research labs. The powder is not normally classified as hazardous, but it remains chemical waste. The working solutions are another matter, because those made up in isopropanol are flammable and form a precipitate on standing, which can mean a clear powder entry and a very different liquid entry sitting in the same cupboard.
- CAS number
- 1320-06-5
- Form
- Solid
- Storage class
- General
- Usual EWC code, laboratory stock
- 16 05 09
What to do this week
- Without moving anything that looks damaged, check the label is readable and write down the opened date if it is not already there.
- Check it is stored as general stock, and apart from anything listed under "Do not store with" on this page. If nothing is listed, that does not prove it is safe with everything.
- If you no longer need it, add it to your disposal list so it can be included in a collection.
Safe storage in a school or lab
On a histology bench the oil red O bottle is most often the isopropanol solution, which is flammable and belongs in a ventilated fire-resistant cabinet away from ignition sources and oxidisers. The powder jar is general stock and needs only a dry, labelled shelf. Keep the two in different places and make sure each carries its own label, because the powder jar's wording does not describe the liquid. Where solutions are made up in batches, date each bottle when it is filled and name the solvent. A tray under each bottle holds any drip and shows red at once on white shelving.
How to tell it has deteriorated
Look at the label and condition of the container first, since the red stain gives leaks away. A red crust at the cap of a solution bottle, a lower level than the record and a stained label show solvent has been escaping through the closure. Settling is expected in these solutions: a precipitate forms on standing, seen as sediment or red flecks, and a bottle with heavy deposit should be listed rather than shaken and reused. Powder that is damp or caked has taken in moisture. A missing date, a faded label or an unidentified red bottle is enough to treat it as unknown.
How it is disposed of
We arrange this by specialist collection. We do not publish a drain or general waste route for any chemical. It is normally packed in a non-regulated or low hazard laboratory pack with compatible chemicals.
Never pour solution down a sink or tip powder into the general bin, and do not leave bottles of isopropanol solution near heat while waiting for collection. The powder is not normally classified as hazardous, yet still counts as chemical waste, and a school, university or laboratory store would normally use 16 05 09 for it, with 18 01 07 where a human healthcare service is clearing it. Solutions are described separately with their solvent and size, because a flammable liquid is packed differently from a dry dye jar.
| Usual EWC code, laboratory stock | 16 05 09 |
|---|---|
| Human healthcare setting | 18 01 07 |
| Usually classed as hazardous waste | No |
The code depends on where the waste came from and what it contains, and classifying it is the producer's duty. Use the waste code finder to see why.
Common quantities
Usually held as: solid.
Tell us the container size and how many you hold. An estimate is enough to start with, and some items cannot be priced until someone has looked at them.
Where it is commonly held
Questions
Is oil red O flammable?
The powder alone is not normally classified as hazardous, but solutions made up in isopropanol are flammable. Store those in a ventilated fire-resistant cabinet away from ignition sources and describe the solvent on the request.
Why is there a red sediment in our working solution?
A precipitate forms on standing in these solutions. Do not shake and reuse a heavily affected bottle. Note the date and list it for collection.
Do we list the powder and solution together?
No. The powder is a dry jar and the solution is a liquid in a solvent, so each needs its own line with container size and condition.
Which waste code do we use for a pathology lab?
A human healthcare setting would normally use 18 01 07 for the powder, whereas a school, university or laboratory store would normally use 16 05 09. State the setting when you request collection.
Related chemicals
Search the chemical database- 1,1,1-TrichloroethaneCAS 71-55-6General
- 1-NaphtholCAS 90-15-3General
- 2-NaphtholCAS 135-19-3General
- 3,3',5,5'-TetramethylbenzidineCAS 54827-17-7General
- 4-NitrophenolCAS 100-02-7General
- AcetophenoneCAS 98-86-2General