Safranin O (CAS 477-73-6): storage and disposal
Red against the purple of crystal violet, safranin O is the counterstain in the Gram method, which is why a bottle of it turns up in nearly every school and college microbiology cupboard and in many pathology and histology stores. It is classified for serious eye damage, so splashes are the thing to guard against. Its form, powder or Gram stain bottle, and the setting it came from decide how it is described for collection.
- CAS number
- 477-73-6
- Form
- Solid
- Storage class
- General
- Signal word
- Danger
- Usual EWC code
- 16 05 06*
Corrosive
What to do this week
- Check the label is readable and write down the opened date if it is not already there.
- Check it is stored as general stock and apart from the items listed under "Do not store with".
- If you no longer need it, add it to your disposal list so it goes on the next collection.
Safe storage in a school or lab
Safranin is held with the other staining stock, not with the cleaning supplies and not at eye height. Keep powder in its original jar and Gram stain bottles in their kit box, on a shelf low enough that nobody has to reach over their head to lift a bottle. Serious eye damage is the classification, so a bench with eye protection and an eyewash nearby matters more than any special cabinet, since the storage class is general. Date every bottle when it arrives, and keep teaching stock separate from anything used in clinical work. A tray beneath the bottles shows leaks quickly because the red dye marks paper labels and white shelving.
How to tell it has deteriorated
This dye gives few clear signs of age, so the container carries the evidence. A red stain on the cap or label means seepage, a crust at the thread means the solution has dried out there, and powder that has caked or looks damp has taken in moisture. A faded or stained label, a cracked bottle or no record of when it was received make the contents unknown, and unknown stock is the real problem. Take a look at the level too: a bottle that is much lower than its record suggests has been leaking or drying out. Do not open an unlabelled red bottle to see what it contains. List it with a plain description of its colour and size.
How it must be disposed of
By specialist collection only. It never goes down the drain or into general waste. It is normally packed in a non-regulated or low hazard laboratory pack with compatible chemicals.
What to record about the container is simple here: the form, the size, whether the Gram kit is intact and the setting. A prep room or lab store uses 16 05 06* for this dye, and clearing human healthcare stock uses 18 01 06*. Tell the collector if the stock is powder or a ready counterstain, since the two are packed as different lines. Keep bottles upright in their boxes where they are sound. Safranin is not to be tipped into a sink to clear a shelf, and it should not be mixed with other stains to consolidate bottles, because a mixed bottle is harder to describe than two small ones.
| EWC code, laboratory chemicals | 16 05 06* |
|---|---|
| Discarded organic chemical, not a laboratory pack | 16 05 08* |
| Human healthcare setting | 18 01 06* |
| Hazardous waste | Yes |
The code depends on where the waste came from and what it is mixed with. Use the waste code finder to see why.
Common quantities we collect
Usually held as: solid, Gram stain.
Part-used bottles and full containers are both fine. Tell us the size and how many, and an estimate is enough for a first price.
Where it is commonly held
Hazard statements
- H318 Causes serious eye damage.
Precautionary statements
- P264 Wash hands and exposed skin thoroughly after handling.
- P264+P265 Wash hands and exposed skin thoroughly after handling. Do not touch eyes.
- P280 Wear protective gloves, protective clothing, eye protection and face protection.
- P302+P352 IF ON SKIN: Wash with plenty of water.
- P305+P351+P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P305+P354+P338 IF IN EYES: Immediately rinse with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P317 Get medical help.
- P321 Specific treatment (see supplemental first aid instruction on this label).
- P332+P317 If skin irritation occurs: Get medical help.
- P337+P317 If eye irritation persists: Get medical help.
- P362+P364 Take off contaminated clothing. And wash it before reuse.
Substance as classified, dilutions may differ.
Questions
Is safranin O the same stock as the rest of a Gram stain kit?
It comes in the same kit but is a separate chemical with its own classification, which here is serious eye damage. List it as its own line alongside the crystal violet and other bottles rather than as one kit entry.
Can we combine half-empty safranin bottles to save space?
No. Combining bottles makes the contents harder to describe and may mix different strengths or ages. Leave each bottle as it is, labelled and dated, and list them individually for collection.
What should I do with a Gram counterstain with no date on it?
Record it as unknown age, check the cap and label, and list it for collection if it is crusted, stained or no longer matches the stock record. This dye shows few signs of age, so the paperwork matters.
Which code applies to school stock?
A school or college store normally uses 16 05 06* for this dye. Hospital pathology or other human healthcare settings clearing stock use 18 01 06*. The setting decides the entry, not the dye.
Related chemicals
Search the chemical database- 1,1,1-TrichloroethaneCAS 71-55-6General
- 1-NaphtholCAS 90-15-3General
- 2-NaphtholCAS 135-19-3General
- 3,3',5,5'-TetramethylbenzidineCAS 54827-17-7General
- 4-NitrophenolCAS 100-02-7General
- AcetophenoneCAS 98-86-2General