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Chemical collection and stock control for UK schools, universities and hospitals

Triton X-100 (CAS 9002-93-1): storage and disposal

Triton X-100 is a thick, clear detergent liquid used in university and pathology labs to open cells and permeabilise tissue sections for staining. It is usually held as neat stock in a bottle that lasts years. The statements on record are skin, eye and respiratory irritation and H400, very toxic to aquatic life, and that last one is why a spill or a leftover cannot be handled casually.

CAS number
9002-93-1
Form
Liquid
Storage class
General
Signal word
Warning
Usual EWC code
16 05 06*
  • Harmful or irritant pictogramHarmful or irritant
  • Hazardous to the environment pictogramHazardous to the environment

What to do this week

  1. Check the label is readable and write down the opened date if it is not already there.
  2. Check it is stored as general stock and apart from the items listed under "Do not store with".
  3. If you no longer need it, add it to your disposal list so it goes on the next collection.

Safe storage in a school or lab

The hazard that decides where it goes is the aquatic one, so a bottle needs secondary containment as much as a shelf. Keep neat stock upright in a plastic tray on a general shelf, tightly closed, with the cap thread wiped clean. Because the liquid is slow and viscous, it gathers around the neck and runs down the label in long drips, and staff often dispense it clumsily. Pathology and university stores usually keep working bottles of diluted detergent made by someone else beside it, so label the neat stock clearly with its full name and opening date. Keep it away from food and drink areas.

How to tell it has deteriorated

Start with the bottle neck. A sticky ring, a long string of gel on the thread and a label stuck to the shelf are the usual signs of a viscous liquid that has been dispensed many times. The liquid itself should be clear and slightly yellow. A cloudy or hazy bottle, an odd smell, floating material or a bottle that has been topped up from another container should be recorded and not used. Foam in the bottle neck usually means someone has shaken it. The cap often seizes in older bottles where dried detergent has glued the thread, and force can crack the neck.

How it must be disposed of

By specialist collection only. It never goes down the drain or into general waste. It is normally packed in a non-regulated or low hazard laboratory pack with compatible chemicals.

Neat Triton X-100 leaves by specialist collection in a lab pack, kept upright in a leak-proof container with its cap secured. With H400 on record it must never go to a drain, even in what looks like a small amount, as this is the classification that makes it a hazardous waste. The code 16 05 06* applies in a research lab, 16 05 08* is used where it is a discarded organic chemical outside a pack, and 18 01 06* applies in a human healthcare setting. Buffers that contain it are mixtures, so describe them by their label and keep them in their own bottles.

Waste codes and transport details for Triton X-100
EWC code, laboratory chemicals16 05 06*
Discarded organic chemical, not a laboratory pack16 05 08*
Human healthcare setting18 01 06*
Hazardous wasteYes

The code depends on where the waste came from and what it is mixed with. Use the waste code finder to see why.

Common quantities we collect

Usually held as: neat.

Part-used bottles and full containers are both fine. Tell us the size and how many, and an estimate is enough for a first price.

Where it is commonly held

Hazard statements

  • H315 Causes skin irritation.
  • H319 Causes serious eye irritation.
  • H335 May cause respiratory irritation.
  • H400 Very toxic to aquatic life.
Precautionary statements
  • P261 Avoid breathing dust, fume, gas, mist, vapours or spray.
  • P264 Wash hands and exposed skin thoroughly after handling.
  • P264+P265 Wash hands and exposed skin thoroughly after handling. Do not touch eyes.
  • P271 Use only outdoors or in a well-ventilated area.
  • P273 Avoid release to the environment.
  • P280 Wear protective gloves, protective clothing, eye protection and face protection.
  • P302+P352 IF ON SKIN: Wash with plenty of water.
  • P304+P340 IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P305+P351+P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P319 Get medical help if you feel unwell.
  • P321 Specific treatment (see supplemental first aid instruction on this label).
  • P332+P317 If skin irritation occurs: Get medical help.
  • P337+P317 If eye irritation persists: Get medical help.
  • P362+P364 Take off contaminated clothing. And wash it before reuse.
  • P391 Collect spillage.
  • P403+P233 Store in a well-ventilated place. Keep container tightly closed.
  • P405 Store locked up.
  • P501 Dispose of contents and container in accordance with local, regional, national and international regulations.

Substance as classified, dilutions may differ.

Questions

Why can't we put a little Triton X-100 down the sink after staining?

It carries H400, very toxic to aquatic life, so it must never go to a drain, even in small amounts. Collect the bottle or the labelled waste container and arrange specialist collection.

The cap on our old bottle is stuck. What should we do?

Do not force it, since the neck can crack and the viscous liquid spill. Place the bottle in a tray, keep it upright, record it and ask for advice. A leaking old bottle goes into a leak-proof outer container.

Which waste code do we use for Triton X-100 in a pathology lab?

A human healthcare setting uses 18 01 06*, while a research lab outside healthcare normally uses 16 05 06*. Tell the collector which applies to the room, and the code will be confirmed on the note.

Guidance only. Check your own risk assessment and your supplier's safety data sheet.

Hazard data source: PubChem GHS Classification, retrieved 31 Oct 2026. Formula C16H26O2. EC number 264-520-1.

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