Trypsin (CAS 9002-07-7): storage and disposal
Cell culture benches in pathology and histology, and teaching labs in schools, colleges and universities, hold trypsin as a powder and as a solution in small bottles. It is classified as causing skin and serious eye irritation and possible respiratory irritation, and it may cause allergy or asthma symptoms or breathing difficulties if inhaled. The solution and the powder age differently, so the form on the shelf matters.
- CAS number
- 9002-07-7
- Form
- Solid
- Storage class
- General
- Signal word
- Danger
- Usual EWC code
- 16 05 06*
Harmful or irritant
Serious health hazard
What to do this week
- Check the label is readable and write down the opened date if it is not already there.
- Check it is stored as general stock and apart from the items listed under "Do not store with".
- If you no longer need it, add it to your disposal list so it goes on the next collection.
Safe storage in a school or lab
The sign on the bottle tells you the form, and supplier labels often state a cold storage requirement, so check them before choosing a shelf. Keep the powder in a tightly closed, labelled tub in a dry store, because dust is the classified hazard, away from food and drink. Solutions should be dated and stored as the label says, in a laboratory fridge or freezer rather than a food unit. On a pathology bench, keep bottles away from clinical samples and acids that may leak. Do not shake the powder out to measure it near other people.
How to tell it has deteriorated
Powder shows damp as caking or a sticky patch, and may smell stale, but enzymes can lose activity without visible change. Solution bottles show more: cloudiness, flecks or a skin, a changed colour, or a leaking cap. Because trypsin is a protein that can digest itself over time in solution, a bottle held warm or for a long period is the likeliest to be spent. A missing date or label means nobody can say how old it is. Record the form, the appearance and the cap condition, and do not open a cloudy bottle.
How it must be disposed of
By specialist collection only. It never goes down the drain or into general waste. It is normally packed in a non-regulated or low hazard laboratory pack with compatible chemicals.
Powder and solution are grouped and packed as separate items, since a liquid bottle is packed differently from a dry tub. Both leave by specialist collection in UN approved packaging under waste code 16 05 06*, with 18 01 06* in a pathology or histology setting. Because inhaling dust is a classified concern, the jar stays closed and nobody should tip powder out to tidy the load. A drain, sink or general bin is not a route for either form, and a spoiled solution should not be poured away. Record the label wording and approximate quantity.
| EWC code, laboratory chemicals | 16 05 06* |
|---|---|
| Human healthcare setting | 18 01 06* |
| Hazardous waste | Yes |
The code depends on where the waste came from and what it is mixed with. Use the waste code finder to see why.
Common quantities we collect
Usually held as: solid, solution.
Part-used bottles and full containers are both fine. Tell us the size and how many, and an estimate is enough for a first price.
Where it is commonly held
Hazard statements
- H315 Causes skin irritation.
- H319 Causes serious eye irritation.
- H334 May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H335 May cause respiratory irritation.
Substance as classified, dilutions may differ.
Questions
What is the main hazard of trypsin powder?
It may cause allergy or asthma symptoms or breathing difficulties if inhaled, and it is classified as an irritant to skin, eyes and airways. Keep the jar closed, avoid creating dust, and do not sweep up spilled powder dry.
Can a trypsin solution that has been left out be collected?
Yes. A solution held warm or for a long time may be spoiled, with cloudiness or flecks, but it goes by the same route. Keep the cap on, record the condition and date if known, and request collection.
Do powder and solution go on the same entry?
No. They are different forms with different containers, so list each separately with its label wording and approximate quantity. The collector groups them into compatible packs.
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